Journal: Nature communications

Article Title: VSV ∆M51 drives CD8 + T cell-mediated tumour regression through infection of both cancer and non-cancer cells.

doi: 10.1038/s41467-024-54111-6

Figure Lengend Snippet: Fig. 8 | Type I IFN and TNF induce costimulatory marker expression on DCs in human tumours. a Schematic of experiment using human head and neck tumour samples. b Frequency of total DCs (left) and CD141+ DCs (right) in human tumors treated for 24 h ex vivo with media or hTNF/hIFNβ (20 ng/mL hTNF and 50 ng/mL hIFNβ) (n = 4 patient tumours per condition, compiled from 4 independent experiments, two-tailed, paired t test). c–e Expression of HLA-ABC (c), CD80 (d)

Article Snippet: The next day, cells were infected with 1, 5 or 10MOI of VSVΔM51-creGFP in serum free RPMI and incubated for 1 h at 37 °C, followed by removal of VSVΔM51-creGFP-containing media and replacement with complete RPMI containing 2.5 ng/mL recombinant mouse IFNβ (R&D systems, 8234-MB-010).

Techniques: Marker, Expressing, Ex Vivo, Two Tailed Test

Journal: Nature communications

Article Title: Comparative therapeutic efficacy of remdesivir and combination lopinavir, ritonavir, and interferon beta against MERS-CoV.

doi: 10.1038/s41467-019-13940-6

Figure Lengend Snippet: Fig. 5 Therapeutic RDV reduces replication and pathology. Percent starting weight of 10–12-week-old female Ces1c−/−hDPP4 mice infected with 5E + 04 pfu MERS M35C4 and treated with a subcutaneous vehicle for RDV (N = 13) or remdesivir (RDV, 25 mg/kg, N = 14) BID beginning 1 dpi or b vehicle for LPV/RTV-IFNb (N = 15), LPV/RTV-IFNb low (N = 16) or LPV/RTV-IFNb high (N = 16) beginning 1 dpi. Oral vehicle or lopinavir/ritonavir (160/40 mg/kg) was administered orally once daily. IFNb low (1x human equivalent dose of 1.6 MIU/kg) and high (25x human equivalent dose of 40 MIU/kg) or PBS vehicle were administered via subcutaneous injection every other day. Asterisks indicate statistical differences by two-way ANOVA with Tukey’s multiple comparison test. c Lung hemorrhage 6 dpi for all animals in a, b scored on a scale of 0–4, where 0 is a normal pink healthy lung and 4 is a diffusely discolored dark red lung. d MERS-CoV lung titer 6 dpi in mice as described in a, b. Asterisks indicate statistical significance (N group described in a and b, P < 0.05) by one-way ANOVA with Kruskal–Wallis test for (c, d). Data for a–d are compiled from two independent experiments. For the box and whisker plots, the boxes encompass the 25th to 75th percentile, the line is at the median, while the whiskers represent the range. e Representative photomicrographs of MERS-CoV antigen (brown) and hematoxylin stained nuclei (blue) in mouse lung tissue sections from 6 dpi. The black bar is 100 µM.

Article Snippet: Recombinant mouse IFNb protein was purchased from R&D Systems (8234-MB/CF, 1.2 × 109 IU/mg calibrated against Murine IFN-beta WHO International Standard) for the in vivo studies and reconstituted in PBS.

Techniques: Infection, Injection, Comparison, Whisker Assay, Staining